![]() OECTs are sensitive, potentially low cost, and biocompatible as operating at low voltage and can be easily miniaturized or fabricated on flexible substrates (Coppedè et al. Organic electrochemical transistors (OECTs) are a combination of a sensor and an amplifier consisting of a channel made of an organic p-type semiconductor with source and drain contacts and a gate electrode immersed into an electrolytic solution (Battista et al. In particular, the DNA-based biosensors have the advantage of being highly specific when compared to immunosensors and harmless in comparison with viable pathogens cells, which represent a great risk for the operator and require the implementation of procedures for hazardous waste handling and disposal (Manzano et al. Molecular methods like PCR and qPCR reduce the analysis time: nevertheless, new approaches involving the construction of biosensors are required to shorten the assay time and to improve routine food analysis. monocytogenes is an important challenge for food industry to reduce economic losses or recalls of products (Jemmi and Stephan 2006). The current ISO 11290 detection method requires long analysis time related to the enrichment step, the isolation on differential media, and the biochemical tests. Listeria monocytogenes is a pathogen responsible for listeriosis, a disease which causes fatal cases due to ingestion of contaminated food, especially ready-to-eat (RTE) products (smoked salmon, dry-cured ham), dairy products, meat, fish, and vegetables (EFSA 2015a, b EFSA 2016 Katzav et al. Comparison between the Listeria monocytogenes Precis™ method and an OECT biosensor.AFM topographic and surface potential maps of a functionalized gold gate surface. ![]() Textile organic electrochemical transistors functionalized with a specific DNA probe.monocytogenes ATCC 7644, and allowed the specific and rapid detection of L. This assay reached a limit of detection of 1.05 ng/μL, corresponding to 0.56 pM of L. The electrochemical activity on gate electrodes was measured and related to the concentration of DNA extracted from samples and hybridized to the specific capture probe immobilized onto the gold surface of the gate. Atomic force microscopy (AFM) was used to obtain topographic maps of the gold gate. The analyses were performed with culture-based methods, Listeria Precis™ method, PCR, and our textile OECT biosensor which used poly(3,4-ethylenedioxythiophene) (PEDOT):polystyrene sulfonate (PSS) (PEDOT:PSS) for doping the organic channel. This work aims to propose a textile organic electrochemical transistor-based (OECT) biosensor to detect L. Many approaches have been extensively investigated to obtain specific and sensitive methods to detect pathogens in food, but they are often not easy to perform and require trained personnel. Foods contaminated by pathogens are responsible for foodborne diseases which have socioeconomic impacts.
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